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Genetic purity testing of two-line hybrid rice seeds by ultrathin-layer isoelectric focusing of proteins

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To test for inbreds among F1 seeds of two-line hybrid rice (Oryza sativa L.) in the laboratory, ultrathin-layer isoelectric focusing (UTLIEF) of seed proteins was used. One female marker band in the photo-thermo-sensitive genic male sterile rice (cv. Peiai 64S) and one common male marker band in the four restorer lines (cv. G67, Peifu, I9-1 and Minkezhan) were found in the pH range of 5-8. Embryoless half-seeds contained the same male marker proteins as whole seeds and could be used for UTLIEF to test inbreds in the laboratory. The corresponding embryo-containing half-seeds could germinate to form seedlings on vermiculite and seedlings could be transplanted into the field for grow-out tests. The genetic purity determined by UTLIEF of proteins from embryoless half-seeds was confirmed by grow-out tests of the corresponding embryo-containing half-seeds. This work shows that UTLIEF of seed proteins could replace grow-out tests to determine F1 genetic purity of two-line hybrid rice. Using half-seeds, the comparison between laboratory and field tests was more objective and accurate than using whole seeds.
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Document Type: Research Article

Publication date: April 1, 2005

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  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
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