Skip to main content
padlock icon - secure page this page is secure

Quantitative GMO detection in maize (Zea mays L.) seed lots by means of a three-dimensional PCR based screening strategy

Buy Article:

$35.00 + tax (Refund Policy)

Due to the preparation of new EU legislation on the adventitious and unavoidable presence of genetically modified seeds in conventional seed lots, quantification of the contamination of conventional seed lots is becoming more and more important. Nowadays quantification is performed by real-time PCR or by statistically based sub-sampling strategies. Due to the fact that both techniques imply the grinding and homogenisation of a sample, specific properties and characteristics of seeds that are important for quantification are lost.

In some cases information is required on the number of transgenic seeds, the zygosity or the identity of the contaminants. For these purposes we propose an alternative method for quantification based on a combination of a three-dimensional pooling of seeds and a qualitative PCR based screening strategy. The identification of a single transgenic seed among a population of 1000 requires no more than 30 PCR reactions; one for each column (10), row (10) and block (10). The window between which quantification is possible lies between 0% GMO and 0.9% GMO which covers the labelling thresholds of 0.3%, 0.5% and 0.7% proposed by the EU.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Document Type: Research Article

Publication date: April 1, 2005

More about this publication?
  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Membership Information
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more