A rapid germination protocol for mile-a-minute weed, Polygonum perfoliatum L
A method for breaking dormancy and rapidly inducing germination of Polygonum perfoliatum L. achenes was developed through a series of laboratory and greenhouse experiments. Polygonum perfoliatum achenes were subjected to various treatments including mechanical scarification, chemical scarification, inundation, cold-wet stratification (4°C), warm-wet stratification (≈23°C) and combinations of these treatments. After-ripened, stored achenes that were scarified for 60 minutes in 93% sulfuric acid and inundated with deionized water germinated following three to four weeks of stratification at 4°C. A subsequent study showed that post-harvest storage duration ("after-ripening") influenced the results of the germination protocol. Achenes germinated after one week of post-harvest storage at ≈23°C, but a high germination percentage (85 ± 4%) was not achieved until after five weeks of post-harvest storage. Achenes that were stratified in leachate water had lower germination percentages than achenes stratified in deionized water, but inhibition of germination was reduced over time. The results of these experiments suggest that P. perfoliatum achene dormancy is enforced by both coat-imposed and chemical-based mechanisms. Coat-imposed dormancy was overcome by scarifying achenes for 60 minutes in 93% sulfuric acid while chemical-based dormancy was overcome by storing freshly harvested achenes for at least one week at ≈23°C and stratifying the seed in deionized water at 4°C for four weeks.
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Document Type: Research Article
Publication date: October 1, 2004
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