Germination of Cercis siliquastrum seeds in response to gibberellic acid and stratification
Various methods for breaking dormancy and enhancing germination of Cercis siliquastrum seeds were evaluated. Seeds were scarified mechanically, with water at 60 or 80°C for 0, 1, 3, 5 or 7 minutes, or chemically with sulfuric acid (H2SO4) or potassium hydroxide (KOH) for 0, 5, 15 or 30 minutes. Non-scarified seeds or mechanically scarified and imbibed seeds were treated with potassium nitrate (KNO3) (0, 0.1, 0.2, 0.3 or 0.4%), thiourea (0, 0.5, 1, 2, 3 or 4%), or gibberellic acid (GA3) (0, 0.7, 1.4, 2.1 or 2.8 mM), or stratified at 4°C for 0, 4, 8, 12, 16 or 20 weeks. Results revealed that non-scarified seeds did not imbibe or germinate irrespective of the treatment. Imbibition occurred only when the seeds were scarified mechanically or with water at 80°C or H2SO4, but none of the imbibed seeds germinated although seed viability was 80%. Treating imbibed seeds with KNO3 or thiourea failed to induce germination. Imbibed seeds that were treated with 1.4 mM GA3 exhibited 48% germination, whereas those stratified for 16 weeks exhibited 77% germination.
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Document Type: Research Article
Publication date: April 1, 2004
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