Studies on the relationship between the content of total phenolics in exudates and germination ability of maize seed during accelerated aging
Experiments were conducted to evaluate the relationship between the content of total phenolics in maize seed exudates and their germination. Three maize hybrids were studied: ZPSC 677, ZPSC 704 and ZPSC 735. The seeds were subjected to accelerated aging at 44°C and nearly 100% relative humidity. The amount of total phenolic constituents in seed exudates was estimated by direct measuring of absorbance at 284nm and by slightly modified Prussian blue method (Butler 1982). Results obtained with two spectrometric methods of phenolic estimation agreed mutual, although absolute numeric values at 284nm are somewhat lower. All three studied maize hybrids during first five days of accelerated aging showed no changes neither in germination nor in concentration of phenolics in exudates. After that period, a significant decrease in germination and increase in content of total phenolics in exudates was observed. At germination lower than 30% concentration of phenolic compounds gently decreased. The high correlation was obtained between the content of total phenolic compounds in exudates and germination during first 14 days of treatment with correlation coefficients higher than −0,9. Results suggested that method based on the determination of total phenolics in exudates may be useful as a rapid and inexpensive indicator of seed quality.
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Document Type: Research Article
Publication date: April 1, 2004
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