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Assessment of viability of Acer caesium and Ulmus wallichiana seeds through the tetrazolium test

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Assessment of the potential viability of deeply dormant seeds of A. caesium and non-dormant seeds of U. wallichiana was done using the Triphenyal Tetrazolium Chloride (TZ) test, and results correlated with actual viability evaluated by nursery emergence in A. caesium and the laboratory germination test in U. wallichiana. Seeds of both species were subjected to different incubation temperatures, soaking time and concentrations of TZ solution. Direct soaking of pre-moistened seeds in 1.0% TZ solution at 30°C for 16 hours or 0.5% TZ solution at 40°C for eight hours for A. caesium, and 1.0% TZ solution at 30°C for 24 hours for U. wallichiana respectively exhibited good colour intensity and easily recognisable staining patterns and may be appropriate conditions for TZ staining of these species. The differently stained seeds were grouped into six and nine categories in A. caesium and U. wallichiana respectively. Among these, three categories in A. caesium and six categories in U. wallichiana were considered viable on the basis of lowest root mean square values. Correlation analysis revealed that the viability of A. caesium and U. wallichiana seeds was accurately predicted by the TZ test.
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Document Type: Research Article

Publication date: October 1, 2003

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  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
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