A comparison of the carbohydrate composition of soybean (Glycine max (L.) Merr.) seed testa and cotyledons from normal and stone seeds
The carbohydrates from the testa and cotyledons of a single variety of soybeans were extracted and analysed. The variety used produces an unusually high proportion of stone seeds which do not imbibe water after a 24h soaking period. Soak tests on samples from 1997 to 2001 harvests showed a consistently high though variable percentage of stone seeds. The stone seeds from the 1998 and 2000 growing seasons were removed from the normal seeds and the seed testa separated from the cotyledons of both samples. After extracting the protein and simple sugars, the monosaccharide products from acid hydrolysis were determined by HPLC. The results showed differences between the seed testa of the normal compared to the stone seeds. No differences between the corresponding cotyledon carbohydrates. Samples of normal and stone seed testa from the 1998 harvest were fractionated into five component parts according to solubility in hot water, 0.5% ammonium oxalate, producing 2 pectin fractions, 3M potassium hydroxide (KOH) producing 2 hemicellulose fractions and a final residue containing mainly cellulose and lignins. The monosaccharide acid hydrolysis products were determined by HPLC. The results indicated that there were only small differences in the basic hydrolysis products of the complex carbohydrates and no definitive differences between the normal and stone seeds. Fractionation of the complex carbohydrates from seed testa of normal and stone seeds also generated no major differences in the constituent acid monosaccharides found in the acid hydrolysis products.
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Document Type: Research Article
Publication date: October 1, 2003
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