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Identification of the parental lines and hybrids of rice (Oryza sativa L.) using RAPD markers

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Application of RAPD markers for the identification of three rice (Oryza sativa L.) hybrids and their parental lines i.e.CMS female parent (A line), maintainer (B line) and pollen parent (R line), was tested using 17 random oligonucleotides (Operon Technologies, USA). It was possible to distinguish each of these genotypes, following a combination of selected primers. The results are discussed in view of its application for the purpose of Plant Variety Protection and for testing the genetic purity of A line and hybrid seed lots.
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Document Type: Research Article

Publication date: April 1, 2003

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  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
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