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Detergent washing technique for the efficient extraction of teliospores of Tilletia indica from contaminated wheat seeds

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A detergent washing technique was developed for the removal of teliospores of Tilletia indica from wheat seeds. In this method, the seeds were washed with varying concentrations of detergents- sodium dodecyl sulphate (SDS), Tween-20, Triton-X-100 and urea. The best results were achieved with 0.005% SDS, 0.001% Tween-20 and 0.05% Triton-X-100 which recovered 90-95% of teliospores added to seeds. The whole procedure takes 20 min/sample and several samples can be processed at once. This procedure can also be used to reduce or eliminate T. indica spores from seeds before planting. All detergents except Tween-20 also increased the percent germination.
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Document Type: Research Article

Publication date: April 1, 2003

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  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
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