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Open Access Colorimetrical performance estimation of a reference hyperspectral microscope for color tissue slide assessment

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A hyperspectral imaging microscope system (HIMS) was recently designed for color truth establishment of histological tissue slides used for whole slide imaging (WSI) systems color performances assessment. Here, we present the estimation procedure of the colorimetrical performance of the HIMS by measuring the transmittance spectra of spatially uniform neutral density and color filters and deriving the color coordinates in the CIELAB color space. The transmittance and CIELAB results are compared to reference transmittance spectra and subsequent CIELAB coordinates provided by measurement of the same region of interest with a spectroradiometer. To measure the same region of interest, the spectroradiometer is equipped with a fiber probe whose tip is set in one of the eyepiece tubes of the microscope. The CIE 1976 color difference, ΔE* ab, is the metric used for goodness estimation.
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Keywords: CIE LAB coordinates; Colorimetry; Hyperspectral microscope; Spectroscopy; Tissue slides

Document Type: Research Article

Publication date: January 26, 2020

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  • For more than 30 years, the Electronic Imaging Symposium has been serving those in the broad community - from academia and industry - who work on imaging science and digital technologies. The breadth of the Symposium covers the entire imaging science ecosystem, from capture (sensors, camera) through image processing (image quality, color and appearance) to how we and our surrogate machines see and interpret images. Applications covered include augmented reality, autonomous vehicles, machine vision, data analysis, digital and mobile photography, security, virtual reality, and human vision. IS&T began sole sponsorship of the meeting in 2016. All papers presented at EIs 20+ conferences are open access.

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