Identification of Early and Late Replicating Heterochromatic Regions on Platyfish (Xiphophorus maculatus) Chromosomes
The platyfish (Xiphophorus maculates) is one of the best studied lower vertebrates, however cytogenetic data on this species is rather limited. This paper presents advances in platyfish cytogenetics, performed by classical techniques enabling the identification of heterochromatin as well as early and late replicating chromosomal regions. Analysis of chromatin resistant to restriction enzymes (Alu I, Dde I and Hinf I) showed pericentromeric, telomeric and interstitial blocks of heterochromatin. DAPI fluorochrome staining revealed that some of the pericentromeric bands are discrete AT rich clusters of chromatin. Analysis of platyfish DNA replication on the chromosomal level showed heterogeneity of fish heterochromatin regarding its replication time; moreover, the replication banding pattern distinguished pairs of homologous chromosomes.
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Document Type: Research Article
Publication date: 2005
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- Folia biologica is an international quarterly journal that publishes papers on the broad field of experimental zoology, nuclear and chromosome research, and also ultrastructural studies. All papers are subject to peer reviews. Indexed in: ISI Master Journal List, Current Contents, Polish Scientific Journals Contents. I.F. 0.667
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