Carotenoids and Carotenoproteins in Asellus aquaticus L. (Crustacea: Isopoda)
Column (CC), thin-layer (TLC), high-performance liquid (HPLC) and ion-exchange chromatography (IEC), were used to investigate corotenoid and carotenoprotein complexes in Asellus aquaticus specimens from the Narew river. The following carotenoids were found: α-carotene, β-carotene, β-cryptoxanthin, lutein, zeaxanthin, diadinoxanthin, mutatoxanthin, crustaxanthin, echinenone, hydroxyechinenone, phoenicoxanthin, canthaxanthin and astaxanthin. Astaxanthin (37.5%), canthaxanthin (21.4%) and phoenicoxanthin (12.3%) were found in the largest amounts. The total carotenoid content was 13.824 μg g−1 of dry mass. Carotenoprotein complexes containing astaxanthin as the prosthetic group were purified from Asellus aquaticus. The carotenoprotein complexes belonged to the crustacyanins group as α- and γ-crustacyanin. The protein forming the α-crustacyanin contained large amounts of such amino-acids as asparic acid, glutamic acid and leucine, whereas the protein of the γ-crustacyanin contained primarily glutamic acid, glycine and lysine.
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Document Type: Research Article
Publication date: 2005
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- Folia biologica is an international quarterly journal that publishes papers on the broad field of experimental zoology, nuclear and chromosome research, and also ultrastructural studies. All papers are subject to peer reviews. Indexed in: ISI Master Journal List, Current Contents, Polish Scientific Journals Contents. I.F. 0.667
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