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Open Access Determination of selenium in clinical plasma samples related to atopic dermatitis study by chronopotentiometric stripping method

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Galvanostatic stripping chronopotentiometry (GSC) was developed and applied for the determination of selenium in human plasma. In this work GSC based on composite carbon electrode coated by a gold layer was optimized concerning various electrochemical parameters (coating procedure, electrolysis potential, electrolysis time, dissolution current). Along with this, the sample preparation was optimized with respect to mineralization conditions (type and concentration of decomposition agent, temperature, time). The human plasma samples mineralized in an autoclave under the optimized conditions (160 °C, 100 min, 22 mol/l HNO3) were appropriately diluted by background electrolyte solution (0.100 mol/l H2SO4 + 0.001 mol/l HCl) and directly analyzed by the optimized GSC method. The proposed method was characterized by excellent performance parameters, the limit of detection was 0.2 ng/ml, accuracy<5%, reproducibility<4%. The proposed method was applied for the investigation of the relationship between atopic dermatitis and selenium concentration in human plasma. Here, patients suffering from atopic dermatitis were monitored during their treatment with a pharmaceutical preparation containing inorganic selenium (Zinkosel®). After six months therapy increased levels of selenium in plasma were detected in 76% of the patients with an improvement of the clinical state in 65% of the patients.
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Document Type: Research Article

Affiliations: 1: Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University, Bratislava, Slovakia 2: Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University, Odbojárov 10, Bratislava, SK-832 32, Slovakia, Email: [email protected]

Publication date: May 1, 2010

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  • Pharmazie is a leading journal in the field of pharmaceutical sciences. As a peer-reviewed scientific journal, Pharmazie is regularly indexed in the relevant databases like Web of science, Journal Citation Reports and many others. The journal is open for submissions from the whole spectrum of pharnaceutical sciences including Pharmaceutical Chemistry, Experimental and Clinical Pharmacology, Drug Analysis, Pharmaceutics, Pharmaceutical Biology, Clinical Pharmacy etc.
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