Transport evaluation of alendronate across Caco-2 cell monolayers
The transport of alendronate through Caco-2 monolayers in the absence and presence of absorption enhancers (sodium taurocholate-STC and dimethyl-β-cyclodextrin-DM-β-CD) was studied. The viability of Caco-2 cells was determined by MTT assay. The effects of the experiment period
and serum existence in Dubelco's Modified Eagle's Medium (DMEM) on cell viability were examined. The least toxic concentrations of alendronate, STC and DM-β-CD were found as 0.2% (w/v), 5 mM and 0.3% (w/v), respectively. Transport experiments were performed with
these concentrations in DMEM supplemented with serum for an 8 h period. DM-β-CD increased the transport of alendronate through Caco-2 monolayers significantly. No significance was observed with STC. Cell integrity was determined by measuring the electrical resistance values at the end
of the transport experiments and found to be decreased to a greater extent with DM-β-CD. These results indicate that DM-β-CD is a promising agent for improving the transport of alendronate.
Document Type: Research Article
Affiliations: 1: Department of Pharmaceutical Technology, Gazi University, Faculty of Pharmacy, Etiler, Ankara, Turkey 2: Department of Pharmaceutical Technology, Gazi University, Faculty of Pharmacy, Etiler, Ankara, 06330, Turkey, Email: [email protected] 3: Food and Mouth Diseases Institute, Ankara, Turkey
Publication date: 01 February 2009
- Pharmazie is a leading journal in the field of pharmaceutical sciences. As a peer-reviewed scientific journal, Pharmazie is regularly indexed in the relevant databases like Web of science, Journal Citation Reports and many others. The journal is open for submissions from the whole spectrum of pharnaceutical sciences including Pharmaceutical Chemistry, Experimental and Clinical Pharmacology, Drug Analysis, Pharmaceutics, Pharmaceutical Biology, Clinical Pharmacy etc.
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