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Open Access HPLC analyses and pharmacokinetic studies of baicalin and oxymatrine in rabbits

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In the present study two simple RP-HPLC methods were developed to determine baicalin and oxymatrine in rabbit serum. Separation was performed on a DiamonsilTM C18 column (200 mm × 4.6 mm I.D., 5 μm) with UV detector at 277 nm for baicalin and 220 nm for oxymatrine. The mobile phase was methanol-water-phosphoric acid 50 : 50 : 0.2 v/v for baicalin and acetonitrile-water (20 : 80, v/v, 5 mmol/L sodium octanesulfonate was contained and pH was adjusted to 3.2 with phosphoric acid for oxymatrine. p-Nitrobenzoic acid and phenacetin were used as internal standards for baicalin and oxymatrine, respectively. The standard curves were linear from 0.5 to 200.0 mg/L for baicalin and from 0.5 to 100.0 mg/L for oxymatrine with correlation coefficients of 0.9994 and 0.9965, respectively. The intra-day and inter-day RSD were less than 5.4% and 7.2% for baicalin and 6.6% and 13.8% for oxymatrine. The mean recoveries were 100.1% for baicalin and 99.1% for oxymatrine. The methods were applied to a pharmacokinetic study of baicalin and oxymatrine in rabbits. The pharmacokinetic parameters were determined after intravenous injections of baicalin and oxymatrine (40 mg/kg) separately and together to rabbits. They all fit to the two-compartment open model. Student's t test shows that there is no significant difference in the main pharmacokinetic parameters including AUC0–∞, when α and β, when baicalin and oxymatrine were administered separately or together.
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Document Type: Research Article

Affiliations: 1: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, People's Republic of China 2: School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, People's Republic of China, Email: [email protected]

Publication date: September 1, 2003

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  • Pharmazie is a leading journal in the field of pharmaceutical sciences. As a peer-reviewed scientific journal, Pharmazie is regularly indexed in the relevant databases like Web of science, Journal Citation Reports and many others. The journal is open for submissions from the whole spectrum of pharnaceutical sciences including Pharmaceutical Chemistry, Experimental and Clinical Pharmacology, Drug Analysis, Pharmaceutics, Pharmaceutical Biology, Clinical Pharmacy etc.
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