Ammi visnaga L. by addition of elicitors
Stimulation of furanochromone accumulation in callus cultures of
In order to check the possibility of producing secondary metabolites, in vitro cultures of A. visnaga callus were established. The best growth of A. visnaga callus was obtained on Murashige and Skoog medium (MS) containing 6-benzyladenine (BA) and α-naphtaleneacetic
acid (NAA). The study was concentrated on the induction of production of secondary metabolites by exposing callus to abiotic elicitors: benzo(1,2,3)-thiadiazole-7-carbothionic acid S-methyl ester (BION®) and a suspension of silica (SiO2) and biotic elicitors: autoclaved
lysates of Enterobacter sakazaki and scleroglucan. GC analysis indicated that not-elicited callus of A. visnaga grown in darkness accumulated 2 times more visnagin than the one which was grown under a 16-h photoperiod. The highest accumulation of visnagin was observed in the
callus culture elicited with scleroglucan or BION®. Scleroglucan induced also the accumulation of khellin in A. visnaga callus. The presented work shows that biosynthesis of pharmacologically important secondary metabolites in A. visnaga cultures could be stimulated by application
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Document Type: Research Article
Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland
Faculty of Chemistry, University of Gdańsk, Gdańsk, Poland
Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, 24 Kładki Street, Gdańsk, PL-80-822, Poland, Email: [email protected]
Publication date: August 1, 2003
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