HPLC analysis of neo-clerodane diterpenoids from Teucrium chamaedrys
A simple, rapid analytical method for the quantitative determination of nine neo-clerodane diterpenoids was developed. The neo-clerodane diterpenoids present in the plant material and extracts were separated with an acetonitrile-water gradient at a flow rate of 1 mL per
minute. The HPLC separation was performed on a Phenomenex Luna C18(2) (150 × 4.6 mm I.D., particle size 5 μm) reversed phase column with detection at 220 nm. The limit of detection was 0.24–0.90 μg/mL. The relative standard deviation (RSD) values for the determination of
neo-clerodane diterpenoids in plant extracts were less than 3.20%. This is the first analytical method developed for qualitative and quantitative analysis of nine neo-clerodane diterpenoids by HPLC with PDA detection.
Document Type: Research Article
Affiliations: 1: Research Institute of Pharmaceutical Sciences, The University of Mississippi, Mississippi, USA 2: Research Institute of Pharmaceutical Sciences, The University of Mississippi, P.O. Box 8048, Mississippi, MS 38677, USA, Email: [email protected] 3: Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, Mississippi, USA
Publication date: 01 July 2003
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