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Responses of the Buds of Three South African Sweet Potato (Ipomoea Batatas) Accessions to Different Cryoprotectants

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BACKGROUND: Plant Vitrification Solution 2 (PVS2) was reported to be a successful cryoprotectant solution in regenerating plantlets from sweet potato shoot tips after cryostorage. However, this was not the case for the South African sweet potato accessions. OBJECTIVE: This study assessed the responses of buds of sweet potato to different concentrations of the components of PVS2 both individually and in combination. MATERIALS AND METHODS: The percentage material that regenerated into plantlets was recorded against the total that was evaluated. RESULTS: Regeneration of buds after treatment with 5% dimethyl sulphoxide was significantly better (p<0.001) than 10-15% dimethyl sulphoxide while exposure to ethylene glycol concentrations of 5-10% were better than 15% for all but one accession. Significant difference (p<0.001) was observed in treatment with 30-40% glycerol. Combination of the components at 5% (PVS5%) was better than 10-15% dimethyl sulphoxide and ethylene glycol and 20% glycerol. CONCLUSION: It is recommended that PVS5% be used for further development of the cryopreservation protocol for the South African accessions.
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Keywords: AXILLARY BUDS; DIMETHYL SULPHOXIDE; ETHYLENE GLYCOL; GLYCEROL; PLANT VITRIFICATION SOLUTION; SWEET POTATO

Document Type: Research Article

Publication date: November 1, 2019

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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