BACKGROUND: The quality of cryopreserved buffalo semen is low due to high susceptibility of sperm membranes to cold shock. OBJECTIVE: The present study was designed to investigate the effect of recombinant type-III antifreeze protein (rAFPIII) from the eelpout (Macrozoarces
americanus) on freezability of buffalo semen. MATERIALS AND METHODS: Semen was collected from three buffalo bulls for three weeks (replicates). Qualified ejaculates (N=18) were split into four aliquots and diluted in Tris-citric acid extender containing 0.0, 0.1, 1 and 10
μg mL-1 of rAFPIII. Semen was cooled to 4 °C, evaluated for sperm motility and PMI, cryopreserved and assessed for post-thaw quality. RESULTS: Supplementation of the extender with rAFPIII didn't affect motility and PMI of chilled semen. Post-thaw sperm motility and
PMI were higher in extender supplemented with rAFPIII (10μg mL-1) compared to control. Sperm viability and acrosome integrity remained the same. CONCLUSION: Addition of rAFPIII in extender improved motility and PMI of cryopreserved buffalo semen.
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Document Type: Research Article
November 1, 2019
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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.