BACKGROUND: Embryo vitrification induces zona hardening, which can further influence the pregnancy outcome after vitrified human embryo transfer. However, the factors in fertilization or cryopreservation that contribute to zona hardening and influence the efficacy of laser zona
thinning (LZT) is not well studied. OBJECTIVE: To investigate whether fertilization methods and vitrification affect the efficacy of LZT treatment. MATERIALS AND METHODS: The present study is a retrospective analysis of embryo transfers. The process involves two fertilization
methods before embryo vitrification. LZT is used to deal with zona hardening. The pregnancy outcomes of 1274 embryo transfer cycles were reviewed statistically. RESULTS: LZT treatment on vitrified embryos fertilized with intracytoplasmic sperm injection (ICSI) resulted in a significantly
lower pregnancy rate and live birth rate compared to that by in vitro fertilization (IVF) (43.0% vs 54.3%, P=0.023; 34.4% vs 45.7%, P=0.021). LZT treatment on vitrified embryos fertilized with ICSI had a negative impact on pregnancy rate and live birth rate. CONCLUSION: The efficacy
of LZT treatment is related to the fertilization method rather than vitrification. LZT treatment for zona hardening of the vitrified embryos fertilized with ICSI leads to a lower pregnancy rate and live birth rate.
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INTRACYTOPLASMIC SPERM INJECTION;
LASER ZONA THINNING;
Document Type: Research Article
November 1, 2019
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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.