BACKGROUND : Cryopreservation is a promising plant germplasm preservation technique that provides genetically stable plantlets. OBJECTIVE: To investigate whether the cryopreservation protocol developed for garlic compromises the performance of the cryopreserved plants,
compared to garlic plants grown in the field. MATERIALS AND METHODS: The field performance of cryopreserved garlic plantlets under in vivo conditions was compared with garlic derived from the field. We assessed both net photosynthetic rate, bulb characteristics and the efficiency of
cryotherapy-induced virus eradication. RESULTS: The superiority of the morphological traits of cryopreserved garlic increased gradually with the growth of the garlic plant. Cryopreservation also improved the net photosynthetic rate, bulb diameter (by 13.4-18.2%), bulb weight (by 37.9-50.5%),
and clove number per bulb (by 25.7-63.6%). The highest virus elimination rate due to cryopreservation was 75.0% for onion yellow dwarf virus (OYDV). CONCLUSION: These findings suggest cryopreservation as a promising method for conservation and improvement of garlic.
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NET PHOTOSYNTHETIC RATE;
VIRUS ELIMINATION RATE
Document Type: Research Article
November 1, 2019
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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.