BACKGROUND: Water transport and intracellular ice formation are important processes that relate to cryoinjury of cells upon freezing. To date, no study is reported on the characteristics of water transport and intracellular ice formation in porcine adipose–derived stem
cells (pADSC). OBJECTIVE: To study water transport and intracellular ice formation upon freezing of pADSCs at different cooling rates. MATERIALS AND METHODS: The pADSCs were isolated using collagenase digestion from a subcutaneous adipose tissue of a 28–day–old Landrace
pig. Freeze experiments were performed in a gas tight chamber of cryomicroscopy stage at different cooling rates between 40°C and 150°C. RESULTS: Water permeability coefficient Lpg and the activation energy ELP
decrease with increasing cooling rates
for pADSCs. The probability of intracellular ice formation increases with increasing cooling rates, being 0.35, 0.4 and 0.5 for cooling rates at 20, 30 and 60 °C/min respectively. CONCLUSION: Based on the characteristics of water transport and intracellular ice formation in pADSCs,
slow freezing is perhaps more suitable for pADSC cryopreservation.
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ADIPOSE-DERIVED STEM CELLS;
INTRACELLULAR ICE FORMATION;
Document Type: Research Article
Publication date: July 1, 2018
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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.