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Cryopreservation of Winter-dormant Apple Buds IV: Critical Temperature Variation That Can Compromise Survival

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BACKGROUND: Precise temperature control in several key areas during cryopreservation of dormant, winter apple buds is critical for maximal survival. OBJECTIVE: To consider the effects of pre–harvest temperature, the duration of incubation at –30°C and variation in rewarming rate on survival. MATERIALS AND METHODS: Dormant winter buds of Malus x domestica cultivars were harvested with two different acclimation histories and cryopreserved with variation in incubation time at –30°C. Recovery from LN using slow, intermediate and rapid rewarming was investigated as well as preservation after prolonged storage at –4°C. RESULTS: The effects on survival of preharvest temperature regime and an altered –30°C incubation regime are cultivar dependent and an increase in rewarming rate has a strong negative effect on recovery. CONCLUSION: Post–thaw survival of the winter–dormant buds can be compromised by increased temperature over a period as short as 5 days prior to bud harvest. Varying incubation times at –30°C produce variable, cultivar dependent, survival and moderate increases in rewarming rates can also radically reduce survival.
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Keywords: COOLING RATE; CRYOPRESERVATION; DORMANT BUD; ENVIRONMENTAL TEMPERATURE; MALUS X DOMESTICA; WARMING

Document Type: Research Article

Publication date: July 1, 2018

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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