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Application of Pectin from Rauvolfia Serpentina (l.) Benth to The Cryopreservation of Human Leucocyte Cell Suspensions

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BACKGROUND: Due to their valuable medicinal properties and high physiological activity, plant polysaccharides are currently being extensively studied. OBJECTIVE: The present study aims to investigate rauwolfian (pectin for Rauvolfia serpentina) supplementation on human leukocytes cryopreservation. We determined the characteristics of leukocytes undergoing freezing with pectin at different temperatures. METHODS: Donor leukocytes were frozen under the protection of comprehensive cryoprotectant solution and stored in electrical freezers (-20°C, -40°C, -80°C). RESULT: A regular decrease of all values starting from a higher temperature (-20°) through to the lower temperature (-80°) was identified. The study showed that pectin rauwolfian stimulated both the oxygen-independent and the oxygen-dependent killer response. We also found that the oxygen-dependent neutrophil killer effect was reduced as the storage temperature was lowered. It was determined that the LPO levels in the cells with added pectin-containing solutions remained the same before freezing, while their antioxidant activity positively increased, which is beneficial for neutrophils for their further freezing to -20°C, -40°C and -80°C. CONCLUSIONS: The results of the study make it possible to assume that rauwolfian, a pectin extracted from Rauvolfia serpentina, has an exocellular protectant effect as part of cryopreservative solution on human white blood cells stored at different low temperatures. The versatility of the substance is probably due to the degree of the macromolecule branching, in particular, the structure of carbohydrate side chains, which contain a large number of hydroxyl groups.
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Keywords: ANTIOXIDANT ACTIVITY; CRYOPROTECTIVE; LEUKOCYTES; LIPID PEROXIDATION; NEUTROPHILS; PECTIN; RAUVOLFIA SERPENTINA

Document Type: Research Article

Publication date: January 1, 2018

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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