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(CRYO)Conservation of Ajania Pacifica (Nakai) Bremer et Humphries Shoot Tips Via Encapsulation-Dehydration Technique

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BACKGROUND: Ajania pacifica, a novelty on the horticultural market, is valued as an ornamental and a medicinal plant. Breeding programs led to the creation of numerous cultivars. Therefore, it is important to develop species storage methods. OBJECTIVE: The aim of this study has been to develop an encapsulation-based cryopreservation protocol of Ajania pacifica shoot tips. MATERIALS AND METHODS: Shoot tips of ajania 'Bengo' were precultured on media with different sucrose and ABA concentrations, encapsulated and dehydrated for various periods and stored in liquid nitrogen. After thawing the explants were inoculated on different recovery media. RESULTS: A higher (9%) sucrose concentration and addition of ABA (15 μM) during preculture, followed by 4-hour desiccation (41% moisture content), as well as the application of cytokinins (1.11 μM BA or 1.16 μM KIN) in the post-thawing recovery medium were necessary to provide a high survival rate of ajania 'Bengo' shoot tips. CONCLUSION: Despite good survival of shoot tips of ajania following cryopreservation procedure, the stimulation of their further growth is a problem.
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Keywords: ABSCISIC ACID; CRYOSTORAGE; DESICCATION; SUCROSE; SYNTHETIC SEEDS; TEM

Document Type: Research Article

Publication date: September 1, 2017

More about this publication?
  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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