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Effects of Cryopreservation on Ultrastructural Morphology of White Shrimp (Litopenaeus Vannamei) Sperm

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BACKGROUND: The structural integrity of a spermatozoon is very important for the processes of fertilization and embryo development. OBJECTIVE: To provide valuable data for developing better cryopreservation techniques for shrimp sperm. MATERIALS AND METHODS: Using scanning electron microscopy and transmission electron microscopy, we examined the morphological alteration of Litopenaeus vannamei sperm after cryopreservation with different concentrations of dimethylsulfoxide (DMSO). RESULTS: We found that the damaged post-thaw sperm presented either vesiculated acrosomal contents, wrinkled membranes, perforated membranes, and loss of the acrosomal spike. The seriously damaged sperm showed missing acrosomal spikes, deformed nuclei, burst nuclear membranes, and vacuolated nuclei. In addition, we found that the post-thaw sperm stored with 5% DMSO had the highest viability rate and lowest DNA damage coefficient by eosin-nigrosin staining and comet assay. CONCLUSION: Our results suggested that cryopreservation has deleterious effects on ultrastructural morphology of L. vannamei sperm, especially on acrosomal spikes and membranes.
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Keywords: CRYOPRESERVATION; LITOPENAEUS VANNAMEI; SPERM; ULTRASTRUCTURAL MORPHOLOGY

Document Type: Research Article

Publication date: September 1, 2017

More about this publication?
  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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