The Presence of Zinc in the Mouse Ovary Vitrification Medium: Histological Evaluation and Follicle Growth

BACKGROUND: Cryopreservation of ovary is a relevant option for preserving fertility of cancer patients undergoing chemotherapy. OBJECTIVE: To evaluate the effect of zinc in the vitrification medium on histology and follicle growth. MATERIALS AND METHODS: Mouse ovaries were vitrified in vitrification medium supplemented with 0, 100, 150 or 200 μg/dl zinc, identified as V0, V1, V2 and V3 groups, respectively. Histological evaluation of ovaries was carried out. The isolated pre-antral follicles were cultured. The size and growth of follicles were assessed. RESULTS: The percentage of morphologically normal follicles increased with increasing zinc concentration in the vitrification medium (P < 0.05). Follicle viability was higher in the V3 than V0 group at the beginning and end of culture (P < 0.05). The highest follicle diameter was obtained in the V3 group after 6 days of culture (P < 0.05). CONCLUSION: Supplementation of the vitrification medium with zinc can improve follicle viability and growth.