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Free Content Effect of Solid Medium During Cooled Storage on Stallion Sperm Parameters

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BACKGROUND: Solid storage medium prevents cellular sedimentation, reduces metabolic demand via limiting movement, and avoids the modification of an extender composition in the sedimentary microenvironment. It has been proven to prolong spermatozoa viability in mammalians. OBJECTIVE: This experiment aims to evaluate the effect of cool storage in solid phase extender on stallion sperms. MATERIALS AND METHODS: Semen was collected from 10 Crioulo stallions (n=30) and submitted to treatments: control group (semen extender) and groups with gelatin addition in different concentrations (semen extender + 1%; 2% and 3%). Seminal analyses included motility, mitochondrial functionality, plasma membrane integrity, DNA and acrosome at 0; 24; 48 and 72 hours during cooled storage at 5°C. RESULTS: Motility, mitochondrial functionality, plasma membrane and acrosome integrity declined during storage time, with no statistical difference between treatments. DNA integrity did not significantly change during storage period. CONCLUSION: Solid medium was not harmful and did not improved stallion sperm parameters during cooled storage.

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Keywords: EQUINE; GELATIN; SEMEN; SOLID-PHASE EXTENDER; SPERMATOZOA

Document Type: Research Article

Publication date: September 1, 2015

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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