Effect of Semen Extender Supplementation with Trehalose, Vitamin C and E on Post-Thaw Min Pig Sperm Qualities
OBJECTIVE: To determine whether the extender supplementation with some antioxidants is able to improve the quality of post-thawed sperm of min pig.
MATERIALS AND METHODS: Ejaculated min pig sperm frozen in lactose-egg yolk (LEY) extender supplemented with trehalose, vitamin C and/or water-soluble vitamin E were thawed, and the quality of sperm samples were evaluated.
RESULTS: The quality of post-thawed sperm samples was higher in the groups with supplementation than that of the control group (P < 0.05). Furthermore, the addition of 100 mM trehalose and 25mM water-soluble vitamin E to the freezing extender strongly preserved the quality of the sperm (P < 0.05).
CONCLUSION: Trehalose and water-soluble vitamin E in the freezing extender effectively improved post-thaw qualities of min pig sperm.
Document Type: Research Article
Publication date: September 1, 2015
CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.