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Free Content Determination of Convective Heat Transfer Coefficient at the Outer Surface of a Cryovial Being Plunged into Liquid Nitrogen

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BACKGROUND: Cell survival upon cryopreservation is affected by the cooling rate. However, it is difficult to model the heat transfer process or to predict the cooling curve of a cryoprotective agent (CPA) solution due to the uncertainty of its convective heat transfer coefficient (h). OBJECTIVE: To measure the h and to better understand the heat transfer process of cryovials filled with CPA solution being plunged in liquid nitrogen. MATERIALS AND METHODS: The temperatures at three locations of the CPA solution in a cryovial were measured. Different h values were selected after the cooling process was modeled as natural convection heat transfer, the film boiling and the nucleate boiling, respectively. And the temperatures of the selected points are simulated based on the selected h values. h was determined when the simulated temperature best fitted the experimental temperature. RESULTS: When the experimental results were best fitted, according to natural convection heat transfer model, h1 = 120 W/(m2·K) while due to film boiling and nucleate boiling regimes hf = 5 W/(m2·K) followed by hn = 245 W/(m2·K). These values were verified by the differential cooling rates at the three locations of a cryovial. CONCLUSION: The heat transfer process during cooling in liquid nitrogen is better modeled as film boiling followed by nucleate boiling.
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Keywords: CONVECTIVE HEAT TRANSFER COEFFICIENT; COOLING RATE; CRYOPROTECTIVE AGENT; CRYOVIAL; FINITE ELEMENT METHOD

Document Type: Research Article

Publication date: July 1, 2015

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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