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Home / Cryoletters, Volume 36, Number 4

Free Content The Combined Use of Honey, Garlic (Allium Sativum L.) and Skimmed Milk As an Extender for Chilling Sheep Semen

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Authors: Jerez-Ebensperger, Rodrigo; Gil, Lydia; Gonzalez, Noelia; De Blas, Ignacio

Source: Cryoletters, Volume 36, Number 4, July 2015, pp. 243-251(9)

Publisher: Cryoletters

BACKGROUND: Sugars are the energetic source for sperm to maintain the metabolic process, and the antibiotics slow down sperm degradation. OBJECTIVE: To study the effects of rosemary honey as energy source and cryoprotectant in combination with garlic as a natural antibiotic on the quality of ram spermatozoa upon cooling. MATERIALS AND METHODS: The ejaculates from three rams were evaluated at different times during cooling to determine its post-dilution quality. RESULTS: Glycerol and dimethylformamide in conjunction with honey and garlic significantly improve the survival of spermatozoa. CONCLUSION: The addition of honey and garlic reduces sperm deterioration when stored at 4°C.

Keywords: ACRIDINE ORANGE; GARLIC; HONEY; OXYDNA; RAM; SKIM MILK; SPERMATOZOA

Document Type: Research Article

Publication date: 01 July 2015

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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