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Free Content Cryopreservation of Rainbow Trout Spermatozoa (Onchorhynchus Mykiss) Using Different Cryodiluents

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An experiment was conducted to evaluate the potential of extenders on sperm motility and to delineate the effect of cyodiluent compostion on post thaw motility, fertilization and hatching percentage in rainbow trout (Onchorhynchus mykiss) sperm. The mature males of rainbow trout with an average weight of (340 ± 15) g and average size (28.8 ± 0.6) cm were used. In the trials for testing the suitability of cryoprotectants, DMSO yielded higher motility percentage with extenders Zhang & Liu and 0.6 M Sucrose. Significantly (P<0.05) higher value of % post thaw motility and fertilization was recorded in cryodiluent composition of 8% DMSO and Zhang & Liu. Additionally, composition of 8% DMSO with Zhang & Liu led to significant (P<0.05) increase in percentage hatching. Overall, results indicated that the combination of (8% DMSO + Zhang & Liu) produced best results in terms of percentage post thaw motility, fertilization and hatching.

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Keywords: DMSO; EXTENDER; HATCHING; MOTILITY; SPERMATOZOA

Document Type: Research Article

Publication date: March 1, 2015

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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