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Free Content Evaluation of Different Methods of Cryopreservation of Ehrlich Tumor Cells

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BACKGROUND: The Ehrlich Ascitic Carcinoma (EAC) is an experimental transplantable neoplasm that develops in several species of mice. The maintenance of the tumor occurs in vivo. Thus, freezing the cells would reduce the number of passages between animals, ensuring genetic stability and storage for longs period of experimentation. OBJECTIVE: Search by EAC cryoprotectants. MATERIALS AND METHODS: The combinations of nutrient medium (Tris, hen egg yolk, and DEMEM) and cryoprotective agent (Glycerol, Trehalose and DMSO) on freezing EAC cells and the transplantability after defrosting were evaluated. The cooling was conducted at 2°C/min. until -180°C and the thawing by immersion in water at 37°C. The transplantability was evaluated from cell inoculation in mice for 14 days. RESULTS: The best results were the associations IA (Cryoprotective agent Glycerol 6% and medium containing 3.0% Tris w / v, 1.8% Citric acid w / v, 1.3% D-fructose w / v and 20% hen egg yolk v / v) and IIB (Cryoprotective agent Trehalose 100mM and medium containing 50% coconut water v / v, 25% sodium citrate 5% v / v and 20% hen egg yolk v / v) with 85.2% and 55.1% viable cells, respectively. CONCLUSION: These transplantable cells were efficient for tumor development, therefore demonstrating that this method of cryopreservation is simple and affordable.

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Keywords: ASCITIC TUMOR; CRYOPROTECTIVE AGENT; GLYCEROL; HEN EGG YOLK; TRANSPLANTABILITY; TREHALOSE

Document Type: Research Article

Publication date: March 1, 2015

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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