Skip to main content
padlock icon - secure page this page is secure

Free Content Hydroxyapatite Nanoparticles Improved Survival Rate of Vitrified Porcine Oocytes and Its Mechanism

Download Article:
 Download
(PDF 334.8 kb)
 
BACKGROUND: Cryopreservation of oocyte has become most essential for the long-term conservation and widespread dispersion of animal genetic resources, but the efficiency of oocyte cryopreservation remains low. OBJECTIVE: The present study is to study the effect of hydroxyapatite (HA) nanoparticles on the survival rate of vitrified oocytes and the possible mechanism of cryopreserving oocytes with nanoparticles. MATERIALS AND METHODS: Porcine oocytes were vitrified in cryoprotectant (CPA) with biocompatible HA nanoparticles by Cryotop. The recrystallization of nano-CPA was observed with cryomicroscope. RESULTS: When 0.01%, 0.02%, 0.05% and 0.1% HA nanoparticles were added into vitrification solution, the survival rate of oocytes after in vitro maturation ranged from 25.9% to 35.4%, which is significantly higher than group without HA nanoparticles (14.7%). The microphotographs of oocytes in different solutions during freezing, thawing and melting showed that HA nanoparticles at a certain concentration can hinder the recrystallization of vitrification solution during rewarming. CONCLUSION: We speculate that preventing the recrystallization is the reason that nano-CPA can promote the survival rate of vitrified oocytes.

6 References.

No Supplementary Data.
No Article Media
No Metrics

Keywords: NANOPARTICLES; OOCYTES; RECRYSTALLIZATION; VITRIFICATION

Document Type: Research Article

Publication date: January 1, 2015

More about this publication?
  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more