BACKGROUND: Cryopreservation of P. canaliculus oocytes has not yet been achieved. OBJECTIVE: The present study is to investigate whether the incorporation of: DMSO (0.09%), α-tocopherol (0.1mM) plus taurine (1mM) and ethylenediaminetetraacetic acid (EDTA;
0.1mM), is beneficial during cryopreservation. METHODS: These three additives were incorporated to both the cryoprotectant (CPA) and recovery media, and evaluated in terms of development and oxidative stress at three key stages of cryopreservation: 1) cryoprotectant addition [10% v/v
ethylene glycol plus 0.2M trehalose; final concentration], 2) cooling to –6°C, and 3) cooling to –35°C and liquid nitrogen immersion. RESULTS: Over all treatments (including controls) progressive cryopreservation steps resulted in a decrease in fertilization and
development to D-larvae, an increase in macromolecular oxidative damage markers (protein carbonyls, lipid hydroperoxides and oxidized DNA), and a decrease in enzymatic (superoxide dismutase, catalase, glutathione S-transferase and glutathione reductase) and non-enzymatic antioxidants. CONCLUSION:
Whilst results varied, the major effects of the additives were the improved percentage fertilization and a decrease in macromolecular damage.
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REACTIVE OXYGEN SPECIES;
Document Type: Research Article
January 1, 2015
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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.