BACKGROUND: Fetoplacental tissues including the early chorion contain stem cells with various morphological and functional characteristics .Cultured chorionic cells may be used in perspective therapies of different pathologies. OBJECTIVE: To investigate the effect of cryopreservation
on proliferation and differentiation potential of chorion cell culture (ChCC). MATERIALS AND METHODS: Five freezing programs for ChCC were compared: Program 1, cooling from 25°C down to -30°C at 0.5°C/min; Program 2, cooling from 25°C down to -30°C at 1°C/min;
Program 3, cooling from 25°C down to -10°C at 1°C/min with further cooling down to - 80°C at 10°C/min; Program 4, cooling from 25°C down to -5°C at 1°C/min with further cooling down to -80°C at 10°C/min; Program 5, cooling from 25°C down to -6°C
at 1°C/min with further crystal seeding by adding the surplus nitrogen into the chamber, and cooling down to -80°C at 10°C/min. Viability, adhesion, proliferation and directed differentiation were examined. RESULTS: Freezing program 5 achieved the best result, with the highest
viability, adhesion, proliferation and directed differentiation. CONCLUSION: The data may help establishing better cryopreservation protocols for perspective chorionic cell lines and their further application in biotechnology.
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CHORION CELL CULTURE;
Document Type: Research Article
January 1, 2015
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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.