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Free Content Successful Production of Piglets Derived from Mature Oocytes Vitrified Using OPS Method

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OBJECTIVE: Examination of effect of vitrification solution with or without foetal calf serum (FCS) on the in vitro and in vivo survival of matured pig oocytes. MATERIALS AND METHODS: Exp. 1: oocytes were exposed to vitrification solutions: VSa (15% DMSO, 15% EG, 0.5 M sucrose dissolved in TCM-199 with FCS) or VSb (VSa without FCS). Exp. 2: oocytes were vitrified in VSa or VSb using OPS. A fraction of vitrified oocytes were transferred to 6 synchronised and inseminated recipients. RESULTS: Survival rate after exposure and vitrification was the same for VSa and VSb. Transfer of 48 oocytes vitrified in VSb resulted with two pregnancies and 12 live piglets. Molecular analysis results: eight piglets originated from the surrogate mother's oocytes, four piglets from vitrified oocytes. CONCLUSION: The use of DMSO and EG as cryoprotectants without serum supplementation was advantageous for the in vivo development of vitrified mature porcine oocytes.

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Keywords: IN VIVO; OOCYTE; OPS VITRIFICATION; PIG; SERUM; VIABILITY

Document Type: Research Article

Publication date: January 1, 2015

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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