MALDI-ToF Study of Peptides from Cryopreserved Tissue Fragments

BACKGROUND: Previously it was shown that the extracts of cryopreserved organ fragments, containing tissue specific peptides, stimulates the reparative regeneration. In order to define the mechanism of action, it is necessary to determine the peptidic composition of extracts. OBJECTIVE: To examine molecular-mass distribution of peptides in the extracts of cryopreserved fragments of pigs' heart and spleen as well as piglets' heart and skin. MATERIALS AND METHODS: The extracts were obtained from cryopreserved fragments of porcine organs. MALDI-ToF method was used to study the peptidic composition of the extracts. RESULTS: The most intense mass spectra peaks, which may correspond to single peptidic molecules, were compared with Protein Knowledgebase (UniProtKB) data. A number of recorded peptides in the extracts expresses high biological activity.