BACKGROUND: For the conservation of hybrid aspen germplasm, cryostorage of dormant in vivo buds is a convenient back-up method for field collections. In practice in Finland, bud collection is performed from February to March. OBJECTIVE: The aim of this study was
to assess how this time schedule can be extended without compromising regeneration. In addition, an easily measurable marker for successful cryopreservation was examined. MATERIALS AND METHODS: Timing of cryopreservation was tested from August to February, using dormant buds from both
outdoor and indoor plants. To find a marker, water content and gene expression of hydrid aspens, as well as environmental factors such as temperature, temperature sum, and light period were followed. RESULTS: Cryopreservation was successful from October to February, when, on an average,
at least 75% of the buds regenerated through micropropagation, and there was no difference to non-frozen controls. Significant genotypic variation was observed in October and February, with regeneration rates of 61-100% and 37-98%, respectively. No marker for successful cryopreservation was
found among the studied factors. CONCLUSION: The results provide flexibility for the undertaking of practical work, with a recommendation that cryopreservation can be carried out from November to January – earlier than the current practice.
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Document Type: Research Article
Publication date: September 1, 2014
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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.