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Free Content Effect of Fasudil Hydrochloride on the post-thaw Viability of Cryopreserved Porcine Adipose-Derived Stem Cells

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BACKGROUND: A dipose-derived stem cells (ADSCs) are of interest for regenerative medicine as they are isolated easily and can differentiate into multiple cell lineages. Recently, it was reported that a Rho-associated kinase (ROCK) inhibitor Y-27632 could enhance the post-thaw viability and physiological function of cryopreserved BMSC. OBJECTIVE: The present study is to investigate whether Fasudil hydrochloride (FH, a selective Rho-kinase inhibitor like Y-27632) can exert a similar beneficial effect on the post-thaw viability of cryopreserved ADSCs. METHODS: ADSCs were allotted in three cryopreservation solutions [I:10% (v/v) (DMSO), 30% (v/v) FBS (as a negative control);II:10μM FH, 10% (v/v) DMSO, 30% (v/v) FBS;III:10 μM Y-27632, 10% (v/v) DMSO, 30% (v/v) fetal bovine serum , All groups were frozen using a rapid freezing method and stored at -196° C in liquid nitrogen for 30 days. After thawing and being cultured 24h, viability of ADSCs were detected by MTT assay. RESULT: The MTT assay showed significant differences in the proportion of adherent viable cells over the concentration of Y-27632 and FH, Additionally, FH did not induce morphological changes in the frozen-thawed ADSCs like Y-27632 did. CONCLUSIONS: FH might represent a promising cryopreservation solution in enhancing the post-thaw viability and physiological function of cryopreserved ADSCs and did not chang the cell appearance.

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Keywords: ADIPOSE-DERIVED STEM CELLS (ADSCS); FASUDIL HYDROCHLORIDE; PORCINE; Y27632

Document Type: Research Article

Publication date: 01 September 2014

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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