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Free Content Screening of Transgenic Frost-Resistant Cotton Using a Porous Silicon Biosensing Platform

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BACKGROUND: The genetic screening of exogenous gene expression is critical for the transgenic plant breeding. OBJECTIVE: Here, a novel label-free porous silicon (PSi)-based biosensor is reported for the identification of frost-resistant cotton. METHODS: Changes in optical response signal in the presence of antifreeze protein (AFP) were detected by Fourier transform infrared (FTIR) spectromicroscopy when binding of the target AFP protein with anti-AFP-antibodies was selectively captured on the PSi biosensor. RESULTS: Compared with non-transgenic plants, significant red shifts were observed for transgenic frost-resistant cotton lines except a transgenic line 2. CONCLUSION: The approach is highly efficient with lower cost and high sensitivity for detecting large number of transgenic samples in the trial field simultaneously. This PSi biosensing platform has potential commercial applications for the rapid assessment of transgenic plants in the field.

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Keywords: ANTIBODY; ANTIFREEZE PROTEIN; POROUS SILICON; PROTEIN BIOSENSOR; TRANSGENIC COTTON

Document Type: Research Article

Publication date: 01 January 2014

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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