A large number of clonal isolates of the prymnesiophyte Phaeocystis antarctica have been established at the Alfred Wegener Institute in Bremerhaven, Germany, to address questions on the genetic diversity and ecological response patterns to climate change. However, at present
the wider scientific community cannot access these strains and their longterm conservation, (currently by serial transfer), cannot be assured. Cryopreservation could provide the solution to these issues, as it would guarantee the long-term security of this genetically and ecological invaluable
collection. This study outlines the successful application of conventional approaches and the use of novel, combined non-penetrating and penetrating cryoprotective strategies that have been successfully applied to the different life-stages of this alga.
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Document Type: Review Article
Publication date: November 1, 2013
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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.