Parameter Conversion from Kedem-Katchalsky Model To Two-Parameter Model Revisited

Successful cryopreservation needs to avoid osmotic injury during the addition and removal of cryoprotective agents. The Kedem-Katchalsky model (KK) and more recently the two-parameter model (2P) are applied to study the volumetric responses of cells for process optimization. The difference between two models is the presence of the reflection coefficient (σ) in the KK model. Earlier studies found that the inclusion of σ is unnecessary and in some cases may lead to conceptual errors when the model is applied cell membranes. Since the 2P model is accurate and simple to use, Chuenkhum et al. had derived the numerical values of 2P parameters for many cell types from KK parameters. We noted, however, that the calculation of Chuenkhum et al. needs further verification because they did not take into consideration of the original experimental conditions in their simulations. The present paper presents the corrected dataset for many cell types.