Successful cryopreservation needs to avoid osmotic injury during the addition and removal of cryoprotective agents. The Kedem-Katchalsky model (KK) and more recently the two-parameter model (2P) are applied to study the volumetric responses of cells for process optimization. The difference
between two models is the presence of the reflection coefficient (σ) in the KK model. Earlier studies found that the inclusion of σ is unnecessary and in some cases may lead to conceptual errors when the model is applied cell membranes. Since the 2P model is accurate and simple
to use, Chuenkhum et al. had derived the numerical values of 2P parameters for many cell types from KK parameters. We noted, however, that the calculation of Chuenkhum et al. needs further verification because they did not take into consideration of the original experimental
conditions in their simulations. The present paper presents the corrected dataset for many cell types.
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MEMBRANE HYDRAULIC CONDUCTIVITY;
Document Type: Research Article
Publication date: July 1, 2013
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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.