This work deals with the construction and performance of a measuring system capable of estimating temperature at sufficiently high speed (up to 1000 samples/sec). Due to its simple design and the utilization of standard materials, it could serve to recording the cooling profile of ultra-rapid
procedures. An immersion device was also developed with the purpose of normalize the penetration speed of the sample in the LN2. The device allows also the comparative analysis of different cooling profiles. The system consists of an immersion device of the sample in the cooling
agent, a temperature measurement system developed by Kleihans F and a laptop computer. To test the system, we recorded the cooling profiles of 10 μL of distilled water and 6 M glycerol solution, obtaining a cooling rate of 8732 °C. min-1 and 4441 °C. min-1
respectively. Also we determine a cooling rate of 204.012 °C.min-1during the immersion of the thermocouple assembly in LN2. Although, the same device, with small technical modifications related to the handling of the sample, could be used to evaluate the recovery
from LN2 temperature to room temperature (re-warming).
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Document Type: Research Article
Publication date: July 1, 2013
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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.