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Free Content Desiccation Sensitivity and Cryopreservation of Excised Embryonic Axes of Citrus Suhuiensis cv. Limau Madu, Citrumelo [Citrus Paradisi Macf. × Poncirus trifoliata (l.) Raf.] and Fortunella polyandra

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Excised embryonic axes from seeds of three taxa, namely, Citrus suhuiensis cv. limau madu, Citrumelo (Citrus paradisi x Poncirus trifoliate) and Fortunella polyandra, were desiccated in a laminar airflow, over silica gel, and ultra-rapidly. Desiccation sensitivity (WC50) was estimated for each taxon using the quantal response model. High desiccation tolerance (WC50 = 0.11 g H2O g-1dw) was observed for limau madu embryonic axes desiccated in a laminar airflow and ultra-rapidly (WC50 = 0.10 g H2O g-1dw). Desiccation tolerance was substantially lower (WC50 = 0.19 g H2O g-1dw) for silica gel dehydration. Similarly, high desiccation tolerance (WC50 = 0.15 g H2O g-1dw) was associated with F. polyandra embryonic axes when desiccated in a laminar airflow, while a lower desiccation tolerance (WC50 = 0.17 g H2O g-1dw) was observed with silica gel dehydration. Ultra-rapid desiccation led to the highest desiccation tolerance (WC50 = 0.14 g H2O g-1dw). The dehydration rate, however, had no influence on desiccation tolerance (WC50 ∼ 0.14 g H2O g-1dw) for Citrumelo embryonic axes. After each desiccation period, embryonic axes were directly immersed in liquid nitrogen (LN) followed by rapid rewarming. Normal seedling recovery of 80 to 83% for excised embryonic axes of limau madu was observed for laminar airflow and ultra-rapid dehydration, but for silica gel dehydration, 57% recovery was obtained. Similarly, for Citrumelo, high recoveries of 100% and 97% were obtained from axes desiccated in a laminar airflow and using ultra-rapid dehydration, respectively, whereas a lower value was associated with silica gel dehydration (80%). For F. polyandra, 50% recovery was obtained both for laminar airflow and ultra-rapid dehydration, while much lower recovery (43%) was associated with silica gel dehydration. Regardless of the drying method employed, axis survival percentages following exposure to LN were commensurate with the desiccation sensitivity pattern.

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Keywords: CITRUMELO (PONCIRUS TRIFOLIATA X CITRUS PARADISI); CITRUS SUHUIENSIS CV. LIMAU MADU; CRYOPRESERVATION; DEHYDRATION RATE; DESICCATION SENSITIVITY; EMBRYONIC AXIS; FORTUNELLA POLYANDRA

Document Type: Research Article

Publication date: May 1, 2012

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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