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Free Content Caseinate Protects Stallion Sperm During Semen Cooling and Freezing

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Extenders with a defined composition containing only components with clearly protective effects on sperm during storage would be an advantage. The aims of the present work were to assess whether caseinate, improves cooled and frozen equine semen quality. Semen from six stallions were suspended with four different cooling extenders C1) Kenney extender; C2) 0.6% caseinate; C3) 2.7% caseinate; and C4) C1 + 2.1% caseinate, and frozen extenders: F1) INRA 82 extender; F2) 1.35% caseinate; and F3) 2.7% caseinate. Although there was no significant difference between the motility rate among the cooled (C1:45.0, C2:36.7, C3:38.3 and C4:48.3) and frozen extenders (F1:16.9, F2:21.1 and F3:18.6), significant higher values of sperm velocity variables were observed with the 1.35% caseinate extender compared to the control (VSL: 40.8x18.9 and VAP: 46.8 x 25.0 μm/s), respectively. Caseinate seemed to be responsible for sperm protection during preservation and showed to be as efficient as milk.

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Keywords: CASEIN; CRYOPRESERVATION; EQUINE; EXTENDER; SEMEN; SPERMATOZOA

Document Type: Research Article

Publication date: May 1, 2012

More about this publication?
  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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