Skip to main content
padlock icon - secure page this page is secure

Free Content Cryoprotective Effect of an Insect Antifreeze Protein MPAFP 698 and its Mutants from the Desert Beetle Microdera Punctipennis

Download Article:
 Download
(PDF 811.8 kb)
 
An insect antifreeze protein, Mp AFP698, from the desert beetle Microdera punctipennis, shares 77% similarity with TmTHP (YL-3) from Tenebrio molitor. The predicted tertiary structure for MpAFP698 was modeled as a regular β-helix with TCT motifs arrayed to form the putative ice-binding surface. This model was validated via site-directed mutagenesis. The results suggest that the desert beetle in central Asia has evolved antifreeze proteins similar to those from North America continent. In an additional study, the recombinant MpAFP698 and its mutants were expressed in Escherichia coli strain BL21 (DE3). The thermal hysteresis activity of MpAFP698 was 1.73°C at 1.0 mg/ml. MpAFP698 was also tested by in vitro antifreeze activity assay to evaluate its cryoprotective effect at -20°C. MpAFP698 displays high cryoprotective effect on bacteria cells at freezing temperatures.

27 References.

No Supplementary Data.
No Article Media
No Metrics

Keywords: ANTIFREEZE PROTEIN; MICRODERA PUNCTIPENNIS; SITE-DIRECTED MUTAGENESIS; THERMAL HYSTERESIS

Document Type: Research Article

Publication date: September 1, 2011

More about this publication?
  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more