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Free Content Cryopreservation of Adult Bovine Testicular Tissue for Spermatogonia Enrichment

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To develop a procedure for cryopreservation of adult bovine testis tissue, the effects of dimethyl sulphoxide (DMSO), propylene glycol (PG), ethylene glycol (EG), and their concentrations (v/v), as well as different thawing temperatures, on the cell viability of bovine testis tissue after freezing/thawing were examined. The highest testicular cell viabilities came from the media containing DMSO (85.3 ± 1.2%), PG (82 ± 1.0%) and EG (83.4 ± 1.0%) at 10% concentration respectively. Using 10% DMSO gave significantly higher spermatogonia percentage (61.1 ± 1.2%, P < 0.001) than processing with 10% PG (54.3 ± 0.6%) or 10% EG (55 ± 1.8%) after differential plating. Thawing in water bath of 37 or 97-100°C also provided significantly higher viabilities (85.1 ± 1.0, 85 ± 1.0%, P < 0.01, respectively) and spermatogonia percentages (56.6 ± 2.0, 56.6 ± 2.6%, P < 0.01, respectively) than that thawing at 4°C (23.4 ± 0.8% for total viability, 8.97 ± 1.0% for spermatogonia percentage). Collectively, 10% DMSO and thawing in 37-100°C water baths were appropriate for the cryopreservation of bovine testicular tissue and subsequent spermatogonia enrichment.

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Keywords: BOVINE; CELLULAR VIABILITY; CRYOPRESEVATION; CRYOPROTECTANT; TESTIS TISSUE

Document Type: Research Article

Publication date: September 1, 2011

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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