Passiflora suberosa is a tropical species used as an ornamental, in popular medicine and in improvement programs. The goal of this study was the development of in vitro conservation strategies for this species, including medium-term storage through slow growth, and long-term
storage through cryopreservation using vitrification-based techniques. Plants were maintained under slow growth conditions on ½ MSM or ¼ MSM medium for 12 months without decrease in regrowth ability. The efficiency of vitrification and encapsulation-vitrification protocols was
compared in order to determine the optimal conditions for successful cryopreservation. Several parameters were evaluated, including pregrowth on medium with high sucrose concentrations, type of vitrification solution (PVS2 and PVS3), exposure time to vitrification solutions, and recovery conditions.
The highest recovery was obtained with the encapsulation-vitrification protocol after a pretreatment with 0.3 M sucrose and post-cryopreservation incubation in the dark for 30 days on MSM medium supplemented with 0.44 μM BA.
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Document Type: Research Article
Publication date: September 1, 2011
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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.